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Recombinant Anti-NCX1 antibody [EPR12739] (ab177952) | Abcam
Recombinant Anti-NCX1 antibody [EPR12739] (ab177952) | Abcam

Recombinant Anti-E Cadherin antibody [EPR699] (ab133597) | Abcam
Recombinant Anti-E Cadherin antibody [EPR699] (ab133597) | Abcam

Why do I get smeared bands in my western blot?
Why do I get smeared bands in my western blot?

Why I got dim and diffused LC3B bands in WB?
Why I got dim and diffused LC3B bands in WB?

Sample Preparation and Gel Electrophoresis | Life Science Research | Merck
Sample Preparation and Gel Electrophoresis | Life Science Research | Merck

Smeared protein gels
Smeared protein gels

Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad
Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad

ELECTROPHORESIS
ELECTROPHORESIS

Protein bands are merged in Western blot, as you will see from the ponceau  staining and the final image i attached?
Protein bands are merged in Western blot, as you will see from the ponceau staining and the final image i attached?

Diffused band on Western Blot gel?
Diffused band on Western Blot gel?

5 pain points in ECL detection and how to overcome them | Cytiva
5 pain points in ECL detection and how to overcome them | Cytiva

Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad
Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad

Chemiluminescent Westerns: How Film and Photochemistry Affect Experimental  Results
Chemiluminescent Westerns: How Film and Photochemistry Affect Experimental Results

Western blot trouble shooting
Western blot trouble shooting

What went wrong? A Western Blot Troubleshooting Guide
What went wrong? A Western Blot Troubleshooting Guide

Western Blot diffuse bands - SDS-PAGE and Western Blotting
Western Blot diffuse bands - SDS-PAGE and Western Blotting

5 pain points in ECL detection and how to overcome them | Cytiva
5 pain points in ECL detection and how to overcome them | Cytiva

Diffused/fused bands of Beta actin in western blots?
Diffused/fused bands of Beta actin in western blots?

Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad
Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad

Thermo Scientific Pierce Western Blotting Troubleshooting Poster
Thermo Scientific Pierce Western Blotting Troubleshooting Poster

Western Bizarre - SDS-PAGE and Western Blotting
Western Bizarre - SDS-PAGE and Western Blotting

Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad
Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad

Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad
Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad

Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad
Western Blot Doctor™ — Protein Band Appearance Problems | LSR | Bio-Rad

Expression of aquaporin 1 and aquaporin 4 water channels in rat choroid  plexus - ScienceDirect
Expression of aquaporin 1 and aquaporin 4 water channels in rat choroid plexus - ScienceDirect

Can anyone explain why do we get diffused bands in western blot? and any  trouble shooting techniques?
Can anyone explain why do we get diffused bands in western blot? and any trouble shooting techniques?